Acetate-onutilizing mutants of Neurospora crassa. I. Mutant isolation, complementation studies, and linkage relationships.
نویسندگان
چکیده
Sixty mutants of Neurospora crassa unable to grow on acetate as sole source of carbon, but able to utilize sucrose, were isolated. On the basis of complementation tests, they were divided into seven groups, each group representing a different gene. Six of the genes have been mapped; no two are closely linked. These loci have been designated acu-1 to acu-7. Mutations at four of these loci result in poor germination of ascospores.
منابع مشابه
(structural and Functional Complexity at the Tryptophan-1 Locus in Neurospora Crassa.)
HE tryptophan-I ( t r yp I ) locus is located in linkage group I11 in Neurospora crassa. TATUM and BONNER (1944) and BEADLE and TATUM (1945) reported tryp-I mutant 10575, which could utilise either indole or tryptophan. AHMAD and CATCHESIDE (1960) brought forward evidence that a new group of five anthranilic acid mutants were allelic to 10575. The present paper describes complementation studies...
متن کاملComplementation analysis of linked circadian clock mutants of Neurospora crassa.
A fourth mutant of Neurospora crassa, designated frq-4, has been isolated in which the period length of the circadian conidiation rhythm is shortened to 19. +/- 0.3 hours. This mutant is tightly linked to the three previously isolated frq mutants, and all four map to the right arm of linkage group VII about 10 map units from the centromere. Complementation tests suggest, but do not prove, that ...
متن کاملGenetical and Biochemical Studies of Histidine-requiring Mutants of Neurospora Crassa. Iv. Linkage Relationships of Hist-3 Mutants.
istidine-3 (hist-3) mutants of Neurospora crassa were chosen for detailed TFEhhemical studies, complementation mapping, and intercrosses because they were shown to exhibit deficiencies in one or the other or both of two distinguishable, though apparently closely related types of biochemical functions. Preliminary heterokaryon complementation studies ( WEBBER 1959, 1960; CATCHESIDE 1960) showed ...
متن کامل"No-growth"-Complementation in Forced Heterokaryons from Sorbose-Resistant (Transport-Defective) Neurospora crassa Mutants
Studies on heterokaryons forced between sorbose-resistant (transport-defective) mutans of Neurospora crassa and a sorbose-sensitive (transport-intact) wildtype strain show that mutants sorr A-l and sorr B-57, mapping in separate linkage groups, are recessive to wildtype; a third mutant, not linked to the others, shows intermediate expression of the resistance character for one of two criteria c...
متن کاملMeiosis in Neurospora crassa. I. The isolation of recessive mutants defective in the production of viable ascospores.
A scheme has been devised for efficient isolation of recessive meiotic mutants of Neurospora crassa. These mutants were detected by their reduced fertility or by the abortion of ascospores. Their isolation involved the selection and screening of the strains arising from ascospores disomic (n + 1) for linkage group I (LG I), which bears the mating-type locus. These strains are self-fertile heter...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 95 3 شماره
صفحات -
تاریخ انتشار 1968